NAEJA has assembled a large selection of assays to be used for testing the selectivity and assessing the potential of toxic effects toxicity of your compound (if any). The list includes:
Serine proteases
leukocyte elastase, HLE
leukocyte elastase - porcine (often used for selectivity with HLE)
cathepsin G
thrombin
plasmin
trypsin
chymotrypsin
Cysteine proteases
cathepsin B
cathepsin L
cathepsin K
cathepsin S
calpain I
gingipain R - bacterial enzyme
gingipain K - bacterial enzyme
Metallo protease
MMP 1, collagenase interstitial
MMP 2, gelatinase 72 kDa
MMP 3, stromelysin
MMP 7, matrilysin
MMP 8, neutrophil collagenase
MMP 9, gelatinese 92 kDa
aminopeptidase M
neutral endopeptidase
Cardiovascular / coagulation panel of enzymes
Cholesterol acyl transferase (responsible for cholesterol absorption in the intestines)
Thrombin
Factor VII a
Factor X a
Plasmim
Central nervous system and neurotransmitters enzymes (can be used for cross reactivity screening)
Acetycholinesterase, AChE
MAO - A ( Monoamine Oxidase A )
Active Transport enzymes (can be used for cross reactivity screening)
Na+ K+ ATP-ase
Bioactive Peptides Regulatory Enzymes (can be used for cross reactivity screening)
NEP , Neutral Endopeptidase
Cytochrome P 450 assays, to test for metabolic liabilities
CYP 1A2
CYP 2C9
CYP 2C19
CYP 2D6
Signal transduction pathway assay (to screen for novel compounds or cross reactivity testing)
ß Insulin receptor kinase, human
